【文献标题】 The distinct role of CD73 in the progression of pancreatic cancer
【作者】Liangjing Zhou1，Shengnan Jia1，Yan Chen，et.al
【作者单位】浙江大学（Zhejiang University）
【文献中引用产品】
【关键词】Pancreatic cancer，Progression，CD73，TNFR2，miR-30a-5p
【DOI】doi.org/10.1007/s00109-018-01742-0
【影响因子(IF)】4.14
【出版期刊】 《Journal of Molecular Medicine》
【产品原文引用】
Detection of TNF-α levels in supernatant via ELISA
Supernatant was investigated for levels of TNF-α protein using commercial TNF-α ELISAs (Hengyuan Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.
Western blot
Cells were harvested, and total protein lysates were resolved using 10% sodium dodecyl sulfate–polyacrylamide gels,electroblotted onto a polyvinylidene fluoride membrane,blocked by 5% skim milk, and probed with primary antibodies listed in Table S1. The membrane was then incubated with the secondary antibody and visualized by enhanced chemiluminescence using Kodak X-Omat LS film (Eastman Kodak,Rochester, USA).
