【文献标题】Conductive materials enhance microbial salt-tolerance in anaerobic digestion of food waste: Microbial response and metagenomics analysis
【作者】Jianhao Li , Xiaofeng Xu , Cong Chen , et.al
【作者单位】重庆大学（Chongqing University）
【文献中引用产品】
微生物蛋白酶（Protease）ELISA试剂盒活性
微生物三磷酸腺苷（ATP）ELISA试剂盒活性
微生物乙酸激酶（AK）ELISA试剂盒活性
微生物辅酶F420（F420）ELISA试剂盒活性
【关键词】Anaerobic digestion ,Salinity stress ,Conductive materials ,Direct interspecies electron transfer ,Metabolic reconstruction
【DOI】https://doi.org/10.1016/j.envres.2023.115779
【影响因子(IF)】8.43
【出版期刊】《Environmental Research》
【产品原文引用】
Flow cytometry (BD, AccuriTMC6 plus, USA) was employed with Annexin V-FITC/PI as the fluorescence dye to detect the intact and injured percentage of microbial cells (Gao et al., 2022). Take 10 mL sludge sample into a centrifuge tube, sonicate the sludge sample to produce a single-cell mixture, filter the single-cell mixture with a 40 μm cell filter to remove large particles, wash twice with phosphate buffered solution (PBS), and adjust the cell concentration to 1.0 × 106–1.0 × 108 cells/mL with PBS to make a cell test solution. Then 490 μL of cell test solution was added to a 2 mL centrifuge tube, 5 μL of Annexin V-FITC and 5 μL of PI dye were added in sequence and mixed evenly. Incubate for 15 min at room temperature in the dark, and the cultured samples were tested by flow cytometry. The data of the flow cytometer was analyzed by FlowJo software. Protease, ATP, acetate kinase (AK), and Coenzyme F420 activity were determined by ELISA kits (Hengyuan biological, Shanghai, China). Electron transport system activity (ETS) of AD sludge was found to be an effective indicator of overall microbial respiration activity in the AD process. The ETS was quantified using a modified 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) method and previously described (Tian et al., 2017). The microbial morphology was characterised by scanning electron microscopy (ZEISS Gemini SEM 300, Germany).
完整版PDF文献请咨询在线客服或者电话联系我司业务员！
更多公司福利请关注“恒远生物”微信公众号！
