【文献标题】A novel Pseudorabies virus vaccine developed using HDR-CRISPR/Cas9 induces strong humoral and cellular immune response in mice
【作者】Chen Luo , Qianqian Wang , Ruhai Guo , et.al
【作者单位】南京孝庄大学（Nanjing Xiaozhuang University）
【文献中引用产品】
小鼠伪狂犬病毒GB抗体（PRVGB-Ab）ELISA试剂盒
【关键词】Pseudorabies virus ,GM-CSF ,CRISPR/Cas9 ,Vaccine
【DOI】https://doi.org/10.1016/j.virusres.2022.198937
【影响因子(IF)】6.28
【出版期刊】《Virus Research》
【产品原文引用】
The blood of mice in each group was collected weekly through the orbital venous post immunization, and then the serums were collected by centrifugation at 3000 rpm for 15 min. The PRV specific glycoproteinB (gB) antibodies were evaluated by the commercial ELISA KIT (Shanghai Hengyuan Bioengineering Co., Shanghai, China) following the manufacturers’ instructions. The results were analyzed with P/N ratios, where P and N denote the OD values of the tested and negative control samples, respectively.
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